| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MCM6 (562-821) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MCM6 |
| Gene ID: |
4175 |
| Uniprot ID: |
MCM6_HUMAN |
| Immunogen Region: |
562-821 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2). |
| Immunogen Sequence: |
SIDRVYSLDDIRRYLLFARQ FKPKISKESEDFIVEQYKHL RQRDGSGVTKSSWRITVRQL ESMIRLSEAMARMHCCDEVQ PKHVKEAFRLLNKSIIRVET PDVNLDQEEEIQMEVDEGAG GINGHADSPAPVNGINGYNE DINQESAPKASLRLGFSEYC RISNLIVLHLRKVEEEEDES ALKRSELVNWYLKEIESEID SEEELINKKRIIEKVIHRLT HYDHVLIELTQAGLKGSTE |
| Post Translational Modifications | O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. |
| Protein Name | Dna Replication Licensing Factor Mcm6P105mcm |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-176974Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962Reactome: R-HSA-69052 |
| Cellular Localisation | NucleusChromosomeBinds To Chromatin During G1 And Detaches From It During S Phase |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm6 antibodyAnti-P105mcm antibodyAnti-MCM6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/176827/367792/STJ11104182_1__60361.1713133337.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit monoclonal antibody (STJ11104182) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
