Anti-MCM3AP antibody (1841-1890 aa) (STJ93212)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GANP at the amino acid range 1841-1890
General Information
| Short Description | Rabbit polyclonal anti-Germinal-center associated nuclear protein (1841-1890 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCM3AP |
| Gene ID | 8888 |
| Uniprot ID | GANP_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GANP at the amino acid range 1841-1890 |
| Immunogen Region | 1841-1890 aa |
| Specificity | GANP Polyclonal Antibody detects endogenous levels of GANP protein. |
Additional Info
| Function | Isoform GANP: As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations. Isoform MCM3AP: Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations. |
| Protein Name | Germinal-Center Associated Nuclear ProteinGanp80 Kda Mcm3-Associated ProteinMcm3 Acetylating ProteinMcm3apMcm3 Acetyltransferase |
| Database Links | |
| Cellular Localisation | Isoform Ganp: Nucleus EnvelopeNucleusNuclear Pore ComplexNucleoplasmChromosomePredominantly Located At The Nuclear EnvelopeFacing The Nucleus InteriorLocalization At The Nuclear Pore Complex Requires Nup153Tpr And Alyref/AlyAlso Found Associated With ChromatinIn B-CellsTargeted To The Immunoglobulin Variable Region GenesIsoform Mcm3ap: CytoplasmTranslocates Into The Nucleus In The Presence Of Mcm3Associates With Chromatin Possibly Through Interaction With Mcm3 |
| Alternative Antibody Names | Anti-Germinal-Center Associated Nuclear Protein antibodyAnti-Ganp antibodyAnti-80 Kda Mcm3-Associated Protein antibodyAnti-Mcm3 Acetylating Protein antibodyAnti-Mcm3ap antibodyAnti-Mcm3 Acetyltransferase antibodyAnti-MCM3AP antibodyAnti-GANP antibodyAnti-KIAA0572 antibodyAnti-MAP80 antibody |
Information sourced from Uniprot.org