• Immunofluorescence was carried out on HEK293 cells transduced with Anti-mCherry Rab1a, using Anti-mCherry polyclonal antibody at a 1:100 dilution.
  • Western Blot was carried out on HEK293 cells transfected with myc-mCherry, using Anti-mCherry polyclonal antibody at a 1:1,000 dilution.
  • Immunofluorescence was carried out on HEK293 cells transduced with Anti-mCherry Rab1a, using Anti-mCherry polyclonal antibody at a 1:100 dilution.
  • Western Blot was carried out on HEK293 cells transfected with myc-mCherry, using Anti-mCherry polyclonal antibody at a 1:1,000 dilution.
  • Immunofluorescence was carried out on HEK293 cells transduced with Anti-mCherry Rab1a, using Anti-mCherry polyclonal antibody at a 1:100 dilution.
  • Western Blot was carried out on HEK293 cells transfected with myc-mCherry, using Anti-mCherry polyclonal antibody at a 1:1,000 dilution.

Anti-mCherry antibody (STJ140000)

SKU:
STJ140000
Current Stock:
Host: Goat
Applications: WB, IF, IHC-P, IHC-F, IEM
Reactivity: mCherry, tdTomato, RFP
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS, 20% glycerol and 0.05% sodium azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Storage Instruction: For continuous use, store at 2-8 °C for one-two days. For extended storage, store in-20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Concentration: 3 mg/mL
Isotype: IgG
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by western blot. This antibody (STJ140000) recognizes very well tdTomato and does not recognize GFP (green fluorescent protein).
Immunogen: Purified recombinant peptide produced in E. coli.
Background Goat polyclonal antibody to mCherry (Cherry fluorescent protein).
validated_applications WB, IF, IHC-P, IHC-F, IEM

Information sourced from Uniprot.org

6 months standard