• Anti-mCherry antibody at 1:1000 dilution; 293 cells transfected with myc-mCherry
  • Immunofluorescence – anti-mCherry antibody using MCR cells transduced with mCherry-Rab11a; cells were fixed with methanol and anti-Rab11a at 1:250
  • Immunofluorescence – anti-mCherry antibody using hCEC cells transduced with mCherry-Rab11a; cells were fixed with methanol and anti-Ra11a at 1:250
  • 293 HEK cells transduced with Ad mCherry Rab1a and stained with anti-mCherry
  • Anti-mCherry antibody in COS-7 cells transfected with mCherry-EEA1 at 1:100 dilution
  • Immunogold labeling of RPE, in vivo injected with mCherry expressing vector
  • Immunofluorescence – anti-mCherry antibody using hCEC cells transduced with mCherry-Rab11a; cells were fixed with methanol and anti-Ra11a at 1/250

Anti-RFP antibody (STJ140000)

SKU:
STJ140000

£85.50 - £532.50
Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF/IEM
Reactivity: mCherry/tdTomato/RFP
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:5000
IF 1:50-1:500
IHC-P 1:50-1:500
IHC-F 1:50-1:500
IEM 1:50-1:500
Takahashi TM Hirano A Kanda T et al. Cell Rep Methods 2022 Nov PMID: 36452866
MacDonald AJ Holmes FE Beall C et al. bioRxiv 2019
Al-Osta I Much
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody recognizes very well tdTomato and does not recognize GFP (green fluorescent protein).
Immunogen: Purified recombinant peptide produced in E. coli.
Immunogen Sequence: MTMITPSLHACRSTLEDPRV PVATMVSKGEEDNMAIIKEF MRFKVHMEGSVNGHEFEIEG EGEGRPYEGTQTAKLKVTKG GPLPFAWDILSPQFMYGSKA YVKHPADIPDYLKLSFPEGF KWERVMNFEDGGVVTVTQDS SLQDGEFIYKVKLRGTNFPS DGPVMQKKTMGWEASSERMY PEDGALKGEIKQRLKLKDGG HYDAEVKTTYKAKKPVQLPG AYNVNIKLDITSHNEDYTI
Background Goat polyclonal antibody to mCherry (Cherry fluorescent protein).

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance