• Confocal imaging of U-2 OS cells using MBNL1 Rabbit monoclonal antibody (STJ11102275, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using MBNL1 Rabbit monoclonal antibody (STJ11102275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MBNL1 antibody (289-388) [S5MR] (STJ11102275)

SKU:
STJ11102275

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MBNL1 (289-388) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MBNL1
Gene ID: 4154
Uniprot ID: MBNL1_HUMAN
Immunogen Region: 289-388
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 289-388 of human MBNL1 (Q9NR56).
Immunogen Sequence: IPQAVLPPLPKRPALEKTNG ATAVFNTGIFQYQQALANMQ LQQHTAFLPPVPMVHGATPA TVSAATTSATSVPFAATATA NQIPIISAEHLTSHKYVTQM
Tissue Specificity Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Function Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
Protein Name Muscleblind-Like Protein 1
Triplet-Expansion Rna-Binding Protein
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Granule
Localized With Ddx1
Tial1 And Ybx1 In Stress Granules Upon Stress
Localized In The Cytoplasm Of Multinucleated Myotubes
Colocalizes With Nuclear Foci Of Retained Expanded-Repeat Transcripts In Myotubes From Patients Affected By Myotonic Dystrophy
Alternative Antibody Names Anti-Muscleblind-Like Protein 1 antibody
Anti-Triplet-Expansion Rna-Binding Protein antibody
Anti-MBNL1 antibody
Anti-EXP antibody
Anti-KIAA0428 antibody
Anti-MBNL antibody

Information sourced from Uniprot.org

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