• Western blot analysis of various lysates using MBD3 Rabbit polyclonal antibody (STJ24511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MBD3 antibody (1-255) (STJ24511)

SKU:
STJ24511

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MBD3
Gene ID: 53615
Uniprot ID: MBD3_HUMAN
Immunogen Region: 1-255
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MBD3 (NP_001268383.1).
Immunogen Sequence: MERKSPSGKKFRSKPQLARY LGGSMDLSTFDFRTGKMLMS KMNKSRQRVRYDSSNQVKGK PDLNTALPVRQTASIFKQPV TKITNHPSNKVKSDPQKAVD QPRQLFWEKKLSGLNAFDIA EELVKTMDLPKGLQGVGPGC TDETLLSAIASALHTSTMPI TGQLSAAVEKNPGVWLNTTQ PLCKAFMVTDEDIRKQEELV QQVRKRLEEALMADMLAHVE ELARDGEAPLDKACAEDDD
Function Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to methylated DNA by itself. Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.
Protein Name Methyl-Cpg-Binding Domain Protein 3
Methyl-Cpg-Binding Protein Mbd3
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-427389
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Reactome: R-HSA-9843940
Reactome: R-HSA-9844594
Reactome: R-HSA-9845323
Cellular Localisation Nucleus
Chromosome
Nuclear
In Discrete Foci
Detected On Chromatin
At Promoter Regions Of Active Genes
Alternative Antibody Names Anti-Methyl-Cpg-Binding Domain Protein 3 antibody
Anti-Methyl-Cpg-Binding Protein Mbd3 antibody
Anti-MBD3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance