| Host: |
Rat |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rat monoclonal antibody anti-EB3 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
KT53 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2a |
| Formulation: |
PBS + 0.05% NaN3 |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:100IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAPRE3 |
| Gene ID: |
22924 |
| Uniprot ID: |
MARE3_HUMAN |
| Specificity: |
The antibody detects a 32 kDa* prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells. |
| Immunogen: |
Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3. |
| Tissue Specificity | Predominantly expressed in brain and muscle. |
| Function | Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. |
| Protein Name | Microtubule-Associated Protein Rp/Eb Family Member 3Eb1 Protein Family Member 3Ebf3End-Binding Protein 3Eb3Rp3 |
| Cellular Localisation | CytoplasmCytoskeletonAssociated With The Microtubule NetworkDetected At The Plus End Of Microtubules |
| Alternative Antibody Names | Anti-Microtubule-Associated Protein Rp/Eb Family Member 3 antibodyAnti-Eb1 Protein Family Member 3 antibodyAnti-Ebf3 antibodyAnti-End-Binding Protein 3 antibodyAnti-Eb3 antibodyAnti-Rp3 antibodyAnti-MAPRE3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
