• Western blot analysis of the lysates from HepG2 cells using JIP3 antibody.
  • Western blot analysis of lysates from HeLa cells, using JIP3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using JIP3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of HeLa cells using JIP-3 Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of various cells using JIP-3 Polyclonal Antibody diluted at 1：1000

Anti-MAPK8IP3 antibody (621-670 aa) (STJ93801)

SKU:
STJ93801

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C-Jun-amino-terminal kinase-interacting protein 3 (621-670 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK8IP3
Gene ID: 23162
Uniprot ID: JIP3_HUMAN
Immunogen Region: 621-670 aa
Specificity: JIP-3 Polyclonal Antibody detects endogenous levels of JIP-3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human JIP3 at the amino acid range 621-670
Function The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner. Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation. Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration. Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development. Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation.
Protein Name C-Jun-Amino-Terminal Kinase-Interacting Protein 3
Jip-3
Jnk-Interacting Protein 3
Jnk Map Kinase Scaffold Protein 3
Mitogen-Activated Protein Kinase 8-Interacting Protein 3
Cellular Localisation Cytoplasm
Golgi Apparatus
Cytoplasmic Vesicle
Cell Projection
Growth Cone
Axon
Dendrite
Perinuclear Region
Localized In The Soma And Growth Cones Of Differentiated Neurites And The Golgi And Vesicles Of The Early Secretory Compartment Of Epithelial Cells
Kif5a/B/C-Mediated Transportation To Axon Tips Is Essential For Its Function In Enhancing Neuronal Axon Elongation
Alternative Antibody Names Anti-C-Jun-Amino-Terminal Kinase-Interacting Protein 3 antibody
Anti-Jip-3 antibody
Anti-Jnk-Interacting Protein 3 antibody
Anti-Jnk Map Kinase Scaffold Protein 3 antibody
Anti-Mitogen-Activated Protein Kinase 8-Interacting Protein 3 antibody
Anti-MAPK8IP3 antibody
Anti-JIP3 antibody
Anti-KIAA1066 antibody

Information sourced from Uniprot.org

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