• Confocal imaging of HeLa cells using ERK1/2 Rabbit monoclonal antibody (STJ11102154, dilution 1:50) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using ERK1/2 Rabbit monoclonal antibody (STJ11102154) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human testis using ERK1/2 Rabbit monoclonal antibody (STJ11102154) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using ERK1/2 Rabbit monoclonal antibody (STJ11102154) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using ERK1/2 antibody (STJ11102154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK1/MAPK3 antibody (180-360) [S4MR] (STJ11102154)

SKU:
STJ11102154

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:200-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK1
MAPK3
Gene ID: 5594
5595
Uniprot ID: MK01_HUMAN
MK03_HUMAN
Immunogen Region: 180-360
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 180-360 of human ERK2 (P28482).
Immunogen Sequence: HTGFLTEYVATRWYRAPEIM LNSKGYTKSIDIWSVGCILA EMLSNRPIFPGKHYLDQLNH ILGILGSPSQEDLNCIINLK ARNYLLSLPHKNKVPWNRLF PNADSKALDLLDKMLTFNPH KRIEVEQALAHPYLEQYYDP SDEPIAEAPFKFDMELDDLP KEKLKELIFEETARFQPGYR S
Tissue Specificity
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 1 antibody
Anti-Map Kinase 1 antibody
Anti-Mapk 1 antibody
Anti-Ert1 antibody
Anti-Extracellular Signal-Regulated Kinase 2 antibody
Anti-Erk-2 antibody
Anti-Map Kinase Isoform P42 antibody
Anti-P42-Mapk antibody
Anti-Mitogen-Activated Protein Kinase 2 antibody
Anti-Map Kinase 2 antibody
Anti-Mapk 2 antibody
Anti-MAPK1 antibody
Anti-ERK2 antibody
Anti-PRKM1 antibody
Anti-PRKM2 antibody
Anti-Mitogen-Activated Protein Kinase 3 antibody
Anti-Map Kinase 3 antibody
Anti-Mapk 3 antibody
Anti-Ert2 antibody
Anti-Extracellular Signal-Regulated Kinase 1 antibody
Anti-Erk-1 antibody
Anti-Insulin-Stimulated Map2 Kinase antibody
Anti-Map Kinase Isoform P44 antibody
Anti-P44-Mapk antibody
Anti-Microtubule-Associated Protein 2 Kinase antibody
Anti-P44-Erk1 antibody
Anti-MAPK3 antibody
Anti-ERK1 antibody
Anti-PRKM3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance