Anti-MAP2K5 antibody (1-190) (STJ29033)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MAP2K5 (1-190) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP2K5 |
| Gene ID | 5607 |
| Uniprot ID | MP2K5_HUMAN |
| Immunogen Region | 1-190 |
| Immunogen Sequence | MLWLALGPFPAMENQVLVIR IKIPNSGAVDWTVHSGPQLL FRDVLDVIGQVLPEATTTAF EYEDEDGDRITVRSDEEMKA MLSYYYSTVMEQQVNGQLIE PLQIFPRACKPPGERNIHGL KVNTRAGPSQHSSPAVSDSL PSNSLKKSSAELKKILANGQ MNEQDIRYRDTLGHGNGGTV YKAYHVPSGK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MEK5 (NP_660143.1). |
Additional Info
| Tissue Specificity | Expressed in many adult tissues. Abundant in heart and skeletal muscle. |
| Post Translational Modifications | Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Function | Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 5Map Kinase Kinase 5Mapkk 5Mapk/Erk Kinase 5Mek 5 |
| Database Links | Reactome: R-HSA-198765 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 5 antibodyAnti-Map Kinase Kinase 5 antibodyAnti-Mapkk 5 antibodyAnti-Mapk/Erk Kinase 5 antibodyAnti-Mek 5 antibodyAnti-MAP2K5 antibodyAnti-MEK5 antibodyAnti-MKK5 antibodyAnti-PRKMK5 antibody |
Information sourced from Uniprot.org