• Immunofluorescence analysis of MCF7 cells using MEK5 Rabbit polyclonal antibody (STJ29033). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MEK5 in paraffin-embedded mouse heart using MEK5 Rabbit polyclonal antibody (STJ29033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MEK5 in paraffin-embedded human appendix using MEK5 Rabbit polyclonal antibody (STJ29033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MEK5 in paraffin-embedded rat heart using MEK5 Rabbit polyclonal antibody (STJ29033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MEK5 Rabbit polyclonal antibody (STJ29033) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-MAP2K5 antibody (1-190) (STJ29033)

SKU:
STJ29033

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP2K5 (1-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K5
Gene ID: 5607
Uniprot ID: MP2K5_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MEK5 (NP_660143.1).
Immunogen Sequence: MLWLALGPFPAMENQVLVIR IKIPNSGAVDWTVHSGPQLL FRDVLDVIGQVLPEATTTAF EYEDEDGDRITVRSDEEMKA MLSYYYSTVMEQQVNGQLIE PLQIFPRACKPPGERNIHGL KVNTRAGPSQHSSPAVSDSL PSNSLKKSSAELKKILANGQ MNEQDIRYRDTLGHGNGGTV YKAYHVPSGK
Tissue Specificity Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Post Translational Modifications Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Function Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 5
Map Kinase Kinase 5
Mapkk 5
Mapk/Erk Kinase 5
Mek 5
Database Links Reactome: R-HSA-198765
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 5 antibody
Anti-Map Kinase Kinase 5 antibody
Anti-Mapkk 5 antibody
Anti-Mapk/Erk Kinase 5 antibody
Anti-Mek 5 antibody
Anti-MAP2K5 antibody
Anti-MEK5 antibody
Anti-MKK5 antibody
Anti-PRKMK5 antibody

Information sourced from Uniprot.org

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