| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-MEK2 (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP2K2 |
| Gene ID: |
5605 |
| Uniprot ID: |
MP2K2_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK2 (P36507). |
| Immunogen Sequence: |
MLARRKPVLPALTINPTIAE GPSPTSEGASEANLVDLQKK LEELELDEQQKKRLEAFLTQ KAKVGELKDDDFERISELGA GNGGVVTKVQHRPSGLIMAR |
| Post Translational Modifications | MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1. (Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 2Map Kinase Kinase 2Mapkk 2Erk Activator Kinase 2Mapk/Erk Kinase 2Mek 2 |
| Database Links | Reactome: R-HSA-112411Reactome: R-HSA-170968Reactome: R-HSA-445144Reactome: R-HSA-5210891Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9652169Reactome: R-HSA-9656223 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinMembrane Localization Is Probably Regulated By Its Interaction With Ksr1 |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibodyAnti-Map Kinase Kinase 2 antibodyAnti-Mapkk 2 antibodyAnti-Erk Activator Kinase 2 antibodyAnti-Mapk/Erk Kinase 2 antibodyAnti-Mek 2 antibodyAnti-MAP2K2 antibodyAnti-MEK2 antibodyAnti-MKK2 antibodyAnti-PRKMK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/95937/378188/STJ116970_1__00761.1713145688.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.")
