• Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-MAP2 (N-term) mouse mAb (dilution 1:100).
  • Immunoprecipitation analysis of Hela cell lysates using MAP2 (N-terminus) mouse mAb.
  • Western blot detection of MAP2 (N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.

Anti-MAP2 antibody (STJ99309)

SKU:
STJ99309
Current Stock:
Host: Mouse
Applications: WB, IP, ICC
Reactivity: Human, Mouse
Short Description: Mouse monoclonal primary antibody against MAP2.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 1D7-B9-C8-F6
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG2b
Dilution Range: WB 1:1000
ICC 1:150
Specificity: This antibody detects endogenous levels of MAP2 (N-terminus) and does not cross-react with related proteins.
Uniprot ID: MTAP2_HUMAN
Gene ID: 4133
Gene Symbol: MAP2
Immunogen: Purified recombinant human MAP2 (N-term) protein fragments expressed in E.coli.
Immunogen Region: N-term
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Protein Name Microtubule-Associated Protein 2
Map-2
Alternative Names Anti-Microtubule-Associated Protein 2 antibody
Anti-Map-2 antibody
Anti-MAP2 antibody

Information sourced from Uniprot.org

6 months standard