• Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
  • Western blot analysis of lysate from 293 cells, using MANF Antibody.
  • Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Western blot analysis of 293 cells using ARP Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-MANF antibody (11-60 aa) (STJ96674)

SKU:
STJ96674

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mesencephalic astrocyte-derived neurotrophic factor (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MANF
Gene ID: 7873
Uniprot ID: MANF_HUMAN
Immunogen Region: 11-60 aa
Specificity: ARP Polyclonal Antibody detects endogenous levels of ARP protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human MANF at the amino acid range 11-60
Function Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions.
Protein Name Mesencephalic Astrocyte-Derived Neurotrophic Factor
Arginine-Rich Protein
Protein Armet
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Endoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Lumen
Retained In The Endoplasmic Reticulum (Er)
And Sarcoplasmic Reticulum (Sr) Under Normal Conditions
Up-Regulated And Secreted By The Er/Sr In Response To Er Stress And Hypoxia
Alternative Antibody Names Anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor antibody
Anti-Arginine-Rich Protein antibody
Anti-Protein Armet antibody
Anti-MANF antibody
Anti-ARMET antibody
Anti-ARP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance