Anti-LYPLA1 antibody [SRM14928] (STJ11109658)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal LYPLA1 antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14928 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:10000-1:60000IHC-P, 1:200-1:800ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LYPLA1 |
| Gene ID | 10434 |
| Uniprot ID | LYPA1_HUMAN |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Platelets. |
| Function | Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA. |
| Protein Name | Acyl-Protein Thioesterase 1Apt-1Hapt1Lysophospholipase 1Lysophospholipase ILpl-ILysopla IPalmitoyl-Protein Hydrolase |
| Database Links | Reactome: R-HSA-203615Reactome: R-HSA-9648002 |
| Cellular Localisation | CytoplasmCell MembraneNucleus MembraneEndoplasmic ReticulumShows Predominantly A Cytoplasmic Localization With A Weak Expression In The Cell MembraneNuclear Membrane And Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Acyl-Protein Thioesterase 1 antibodyAnti-Apt-1 antibodyAnti-Hapt1 antibodyAnti-Lysophospholipase 1 antibodyAnti-Lysophospholipase I antibodyAnti-Lpl-I antibodyAnti-Lysopla I antibodyAnti-Palmitoyl-Protein Hydrolase antibodyAnti-LYPLA1 antibodyAnti-APT1 antibodyAnti-LPL1 antibody |
Information sourced from Uniprot.org