| Host: |
Goat |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-LRRK2 (Internal Region) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). Use at a dilution of 1:200 to 1:2000. The optimal dilution should be determined by the end user. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
LRRK2 |
| Gene ID: |
120892 |
| Uniprot ID: |
LRRK2_HUMAN |
| Immunogen Region: |
Internal Region |
| Specificity: |
Specific for Dardarin. |
| Immunogen: |
A synthetic peptide from internal region of human Dardarin (LRRK2, PARK8) conjugated to an immunogenic carrier protein has been used as the antigen. |
| Post Translational Modifications | Autophosphorylated at Ser-1292.autophosphorylation is stimulated by RAB29. Phosphorylation of Ser-910 and either Ser-935 or Ser-1444 facilitates interaction with YWHAG. Phosphorylation of Ser-910 and/or Ser-935 facilitates interaction with SFN. Ubiquitinated by TRIM1.undergoes 'Lys-48'-linked polyubiquitination leading to proteasomal degradation. |
| Function | Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A. Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Phosphorylates PRDX3. By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis. Acts as a positive regulator of innate immunity by mediating phosphorylation of RIPK2 downstream of NOD1 and NOD2, thereby enhancing RIPK2 activation. Independent of its kinase activity, inhibits the proteasomal degradation of MAPT, thus promoting MAPT oligomerization and secretion. In addition, has GTPase activity via its Roc domain which regulates LRRK2 kinase activity. Recruited by RAB29/RAB7L1 to overloaded lysosomes where it phosphorylates and stabilizes RAB8A and RAB10 which promote lysosomal content release and suppress lysosomal enlargement through the EHBP1 and EHBP1L1 effector proteins. |
| Protein Name | Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2Dardarin |
| Database Links | Reactome: R-HSA-8857538 |
| Cellular Localisation | Cytoplasmic VesiclePerikaryonGolgi Apparatus MembranePeripheral Membrane ProteinCell ProjectionAxonDendriteEndoplasmic Reticulum MembraneSecretory VesicleSynaptic Vesicle MembraneEndosomeLysosomeMitochondrion Outer MembraneCytoplasmCytoskeletonPhagosomeColocalized With Rab29 Along Tubular Structures Emerging From Golgi ApparatusLocalizes To Endoplasmic Reticulum Exit Sites (Eres)Also Known As Transitional Endoplasmic Reticulum (Ter)Detected On Phagosomes And Stressed Lysosomes But Not Detected On Autophagosomes Induced By StarvationRecruitment To Stressed Lysosomes Is Dependent On The Atg8 Conjugation System Composed Of Atg5Atg12 And Atg16l1 And Leads To Lysosomal Stress-Induced Activation Of Lrrk2 |
| Alternative Antibody Names | Anti-Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 antibodyAnti-Dardarin antibodyAnti-LRRK2 antibodyAnti-PARK8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
