• Immunofluorescence analysis of U2OS cells using Lamin B2 antibody (STJ28566). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Lamin B2 Antibody (STJ28566) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human adenomyosis using Lamin B2 Antibody (STJ28566) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Lamin B2 Antibody (STJ28566) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Lamin B2 antibody (STJ28566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-LMNB2 antibody (341-620) (STJ28566)

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STJ28566

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB2
Gene ID: 84823
Uniprot ID: LMNB2_HUMAN
Immunogen Region: 341-620
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human Lamin B2 (NP_116126.3).
Immunogen Sequence: AMAGERDKFRKMLDAKEQEM TEMRDVMQQQLAEYQELLDV KLALDMEINAYRKLLEGEEE RLKLSPSPSSRVTVSRATSS SSGSLSATGRLGRSKRKRLE VEEPLGSGPSVLGTGTGGSG GFHLAQQASASGSVSIEEID LEGKFVQLKNNSDKDQSLGN WRIKRQVLEGEEIAYKFTPK YILRAGQMVTVWAAGAGVAH SPPSTLVWKGQSSWGTGESF RTVLVNADGEEVAMRTVKK
Post Translational Modifications B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Phosphorylation plays a key role in lamin organization, subcellular localization and nuclear envelope disintegration. Phosphorylation by CDK1 at Ser-37 and Ser-407 at the onset of mitosis drives lamin disassembly and nuclear envelope breakdown.
Function Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
Protein Name Lamin-B2
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B2 antibody
Anti-LMNB2 antibody
Anti-LMN2 antibody

Information sourced from Uniprot.org

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