Western blot analysis of extracts of various cell lines, using LIPA antibody (STJ28468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-LIPA antibody (110-399) (STJ28468)

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STJ28468
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIPA
Gene ID: 3988
Uniprot ID: LICH_HUMAN
Immunogen Region: 110-399
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 110-399 of human LIPA (NP_001121077.1).
Immunogen Sequence: DAGFDVWMGNSRGNTWSRKH KTLSVSQDEFWAFSYDEMAK YDLPASINFILNKTGQEQVY YVGHSQGTTIGFIAFSQIPE LAKRIKMFFALGPVASVAFC TSPMAKLGRLPDHLIKDLFG DKEFLPQSAFLKWLGTHVCT HVILKELCGNLCFLLCGFNE RNLNMSRVDVYTTHSPAGTS VQNMLHWSQAVKFQKFQAFD WGSSAKNYFHYNQSYPPTYN VKDMLVPTAVWSGGHDWLA
Tissue Specificity Most abundantly expressed in brain, lung, kidney and mammary gland, a moderate expression seen in placenta and expressed at low levels in the liver and heart.
Post Translational Modifications Glycosylation is not essential for catalytic activity.
Function Catalyzes the deacylation of cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol. Hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol) with preference for the acyl moieties at the sn-1 or sn-3 positions.
Protein Name Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase
Acid Cholesteryl Ester Hydrolase
Lal
Cholesteryl Esterase
Diacylglycerol Lipase
Lipase A
Sterol Esterase
Triacylglycerol Ester Hydrolase
Triacylglycerol Lipase
Database Links Reactome: R-HSA-8964038
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase antibody
Anti-Acid Cholesteryl Ester Hydrolase antibody
Anti-Lal antibody
Anti-Cholesteryl Esterase antibody
Anti-Diacylglycerol Lipase antibody
Anti-Lipase A antibody
Anti-Sterol Esterase antibody
Anti-Triacylglycerol Ester Hydrolase antibody
Anti-Triacylglycerol Lipase antibody
Anti-LIPA antibody

Information sourced from Uniprot.org

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