• Western blot analysis of extracts of various cell lines, using LGI1 antibody (STJ11102962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LGI1 antibody (150-250) [S2MR] (STJ11102962)

SKU:
STJ11102962

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LGI1 (150-250) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LGI1
Gene ID: 9211
Uniprot ID: LGI1_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human LGI1 (O95970).
Immunogen Sequence: NLQTLPKDIFKGLDSLTNVD LRGNSFNCDCKLKWLVEWLG HTNATVEDIYCEGPPEYKKR KINSLSSKDFDCIITEFAKS QDLPYQSLSIDTFSYLNDEY V
Tissue Specificity Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1: Expressed in the occipital cortex and hippocampus.higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is between 3 and 5 times more abundant than in the hippocampus (at protein level). Expression is absent in the cerebellum. Isoform 3: Abundantly expressed in the occipital cortex and weakly expressed in the hippocampus (at protein level).
Post Translational Modifications Glycosylated.
Function Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
Protein Name Leucine-Rich Glioma-Inactivated Protein 1
Epitempin-1
Database Links Reactome: R-HSA-5682910
Cellular Localisation Secreted
Synapse
Cytoplasm
Isoform 1: Golgi Apparatus
Isoform 2: Endoplasmic Reticulum
Alternative Antibody Names Anti-Leucine-Rich Glioma-Inactivated Protein 1 antibody
Anti-Epitempin-1 antibody
Anti-LGI1 antibody
Anti-EPT antibody
Anti-UNQ775 antibody
Anti-PRO1569 antibody

Information sourced from Uniprot.org

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