| Host: |
Rabbit |
| Applications: |
IHC |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LEPREL2 (487-736) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
P3H3 |
| Gene ID: |
10536 |
| Uniprot ID: |
P3H3_HUMAN |
| Immunogen Region: |
487-736 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 487-736 of human LEPREL2 (NP_055077.2). |
| Immunogen Sequence: |
DAAGAGARSGYRGRRSPHTP HERFEGLTVLKAAQLARAGT VGSQGAKLLLEVSERVRTLT QAYFSPERPLHLSFTHLVCR SAIEGEQEQRMDLSHPVHAD NCVLDPDTGECWREPPAYTY RDYSGLLYLNDDFQGGDLFF TEPNALTVTARVRPRCGRLV AFSSGVENPHGVWAVTRGRR CALALWHTWAPEHREQEWIE AKELLQESQEEEEEEEEEMP SKDPSPEPPSRRHQRVQDK |
| Tissue Specificity | Detected in fetal cartilage (at protein level). Weak expression in heart, lung, ovary and skeletal muscle. |
| Function | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea. Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly. Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases. |
| Protein Name | Prolyl 3-Hydroxylase 3Leprecan-Like Protein 2Protein B |
| Database Links | Reactome: R-HSA-1650814 |
| Cellular Localisation | Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Prolyl 3-Hydroxylase 3 antibodyAnti-Leprecan-Like Protein 2 antibodyAnti-Protein B antibodyAnti-P3H3 antibodyAnti-LEPREL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MST1/STK4 knockout (KO) HeLa cells, using [KO Validated] MST1/STK4 Rabbit polyclonal antibody (STJ11100073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89143/357593/STJ11100073_1__61039.1713121731.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MST1/STK4 knockout (KO) HeLa cells, using [KO Validated] MST1/STK4 Rabbit polyclonal antibody (STJ11100073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
