| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KRIT1 (467-736) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KRIT1 |
| Gene ID: |
889 |
| Uniprot ID: |
KRIT1_HUMAN |
| Immunogen Region: |
467-736 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 467-736 of human KRIT1 (NP_919438.1). |
| Immunogen Sequence: |
SENLSLQLKPYHKPLQHVRD WPEILAELTNLDPQRETPQL FLRRDVRLPLEVEKQIEDPL AILILFDEARYNLLKGFYTA PDAKLITLASLLLQIVYGNY ESKKHKQGFLNEENLKSIVP VTKLKSKAPHWTNRILHEYK NLSTSEGVSKEMHHLQRMFL QNCWEIPTYGAAFFTGQIFT KASPSNHKVIPVYVGVNIKG LHLLNMETKALLISLKYGCF MWQLGDTDTCFQIHSMENK |
| Tissue Specificity | Low levels in brain. Very weak expression found in heart and muscle. |
| Function | Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1.this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule-associated protein that binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-containing membranes in a GTP-bound RAP1-dependent manner. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels. May play a role in the regulation of macroautophagy through the down-regulation of the mTOR pathway. |
| Protein Name | Krev Interaction Trapped Protein 1Krev Interaction Trapped 1Cerebral Cavernous Malformations 1 Protein |
| Cellular Localisation | CytoplasmCytoskeletonCell MembranePeripheral Membrane ProteinCell JunctionKrit1 And Cdh5 Reciprocally Regulate Their Localization To Endothelial Cell-Cell JunctionsAssociation With Rap1 Relocalizes Krit1 From Microtubules To Cell Junction MembranesTranslocates From The Cytoplasm Along Microtubules To The Cell Membrane In A Itgb1bp1-Dependent Manner |
| Alternative Antibody Names | Anti-Krev Interaction Trapped Protein 1 antibodyAnti-Krev Interaction Trapped 1 antibodyAnti-Cerebral Cavernous Malformations 1 Protein antibodyAnti-KRIT1 antibodyAnti-CCM1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
