• Confocal immunofluorescence analysis of U-2 OS cells using DRP1 Polyclonal Antibody (STJ11104278) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat brain, using DRP1 antibody (STJ11104278) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DNM1L antibody (437-736) (STJ11104278)

SKU:
STJ11104278

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dynamin-1-Like Protein (437-736) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNM1L
Gene ID: 10059
Uniprot ID: DNM1L_HUMAN
Immunogen Region: 437-736
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 437-736 of human DRP1. (NP_036192.2).
Immunogen Sequence: EEMQRIIQHCSNYSTQELLR FPKLHDAIVEVVTCLLRKRL PVTNEMVHNLVAIELAYINT KHPDFADACGLMNNNIEEQR RNRLARELPSAVSRDKSSKV PSALAPASQEPSPAASAEAD GKLIQDSRRETKNVASGGGG VGDGVQEPTTGNWRGMLKTS KAEELLAEEKSKPIPIMPAS PQKGHAVNLLDVPVPVARKL SAREQRDCEVIERLIKSYFL IVRKNIQDSVPKAVMHFLV
Tissue Specificity Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Post Translational Modifications Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 by CAMK1 and PKA inhibits the GTPase activity, leading to a defect in mitochondrial fission promoting mitochondrial elongation. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 by CDK1 and PINK1 activates the GTPase activity and promotes mitochondrial fission. Phosphorylated in a circadian manner at Ser-637. Sumoylated on various lysine residues within the B domain, probably by MUL1. Sumoylation positively regulates mitochondrial fission. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity. S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage. Ubiquitination by MARCHF5 affects mitochondrial morphology. O-GlcNAcylation augments the level of the GTP-bound active form of DNM1L and induces translocation from the cytoplasm to mitochondria in cardiomyocytes. It also decreases phosphorylation at Ser-637.
Function Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane. Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner. Plays an important role in mitochondrial fission during mitosis. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis.this requirement may depend upon the cell type and the physiological apoptotic cues. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles.the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution. Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production. Isoform 1: Inhibits peroxisomal division when overexpressed. Isoform 4: Inhibits peroxisomal division when overexpressed.
Protein Name Dynamin-1-Like Protein
Dnm1p/Vps1p-Like Protein
Dvlp
Dynamin Family Member Proline-Rich Carboxyl-Terminal Domain Less
Dymple
Dynamin-Like Protein
Dynamin-Like Protein 4
Dynamin-Like Protein Iv
Hdyniv
Dynamin-Related Protein 1
Database Links Reactome: R-HSA-75153
Cellular Localisation Cytoplasm
Cytosol
Golgi Apparatus
Endomembrane System
Peripheral Membrane Protein
Mitochondrion Outer Membrane
Peroxisome
Membrane
Clathrin-Coated Pit
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Mainly Cytosolic
Recruited By Rala And Ralbp1 To Mitochondrion During Mitosis
Translocated To The Mitochondrial Membrane Through O-Glcnacylation And Interaction With Fis1
Colocalized With Marchf5 At Mitochondrial Membrane
Localizes To Mitochondria At Sites Of Division
Localizes To Mitochondria Following Necrosis Induction
Recruited To The Mitochondrial Outer Membrane By Interaction With Mief1
Mitochondrial Recruitment Is Inhibited By C11orf65/Mfi
Associated With Peroxisomal Membranes
Partly Recruited There By Pex11b
May Also Be Associated With Endoplasmic Reticulum Tubules And Cytoplasmic Vesicles And Found To Be Perinuclear
In Some Cell Types
Localizes To The Golgi Complex
Binds To Phospholipid Membranes
Alternative Antibody Names Anti-Dynamin-1-Like Protein antibody
Anti-Dnm1p/Vps1p-Like Protein antibody
Anti-Dvlp antibody
Anti-Dynamin Family Member Proline-Rich Carboxyl-Terminal Domain Less antibody
Anti-Dymple antibody
Anti-Dynamin-Like Protein antibody
Anti-Dynamin-Like Protein 4 antibody
Anti-Dynamin-Like Protein Iv antibody
Anti-Hdyniv antibody
Anti-Dynamin-Related Protein 1 antibody
Anti-DNM1L antibody
Anti-DLP1 antibody
Anti-DRP1 antibody

Information sourced from Uniprot.org

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