| Short Description : | Rabbit polyclonal LDHA (1-332) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5ug-4ug antibody for 200ug-400ug extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay req |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LDHA |
| Gene ID: | 3939 |
| Uniprot ID: | LDHA_HUMAN |
| Immunogen Region: | 1-332 |
| Immunogen Sequence: | MATLKDQLIYNLLKEEQTPQ NKITVVGVGAVGMACAISIL MKDLADELALVDVIEDKLKG EMMDLQHGSLFLRTPKIVSG KDYNVTANSKLVIITAGARQ QEGESRLNLVQRNVNIFKFI IPNVVKYSPNCKLLIVSNPV DILTYVAWKISGFPKNRVIG SGCNLDSARFRYLMGERLGV HPLSCHGWVLGEHGDSSVPV WSGMNVAGVSLKTLHPDLGT DKDKEQWKEVHKQVVESAY |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1). |
| Tissue Specificity | Predominantly expressed in anaerobic tissues such as skeletal muscle and liver. |
| Post Translational Modifications | ISGylated. |
| Function | Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+). |
| Protein Name | L-Lactate Dehydrogenase A ChainLdh-ACell Proliferation-Inducing Gene 19 ProteinLdh Muscle SubunitLdh-MRenal Carcinoma Antigen Ny-Ren-59 |
| Database Links | Reactome: R-HSA-70268Reactome: R-HSA-9861718 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-L-Lactate Dehydrogenase A Chain antibodyAnti-Ldh-A antibodyAnti-Cell Proliferation-Inducing Gene 19 Protein antibodyAnti-Ldh Muscle Subunit antibodyAnti-Ldh-M antibodyAnti-Renal Carcinoma Antigen Ny-Ren-59 antibodyAnti-LDHA antibodyAnti-PIG19 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of L929 cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/176542/367362/STJ11103897_1__40674.1713132901.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
