• Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (STJ24413) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (STJ24413) at 1:1000 dilution. L929 cells were treated by staurosporine (1 uM) for 3 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Lamin B1 rabbit polyclonal antibody (STJ24413) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Lamin B1 rabbit polyclonal antibody (STJ24413) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Lamin B1 rabbit polyclonal antibody (STJ24413) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal immunofluorescence analysis of HeLa cells using Lamin B1 Polyclonal Antibody (STJ24413) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Lamin B1 rabbit polyclonal antibody (STJ24413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Lamin B1 rabbit polyclonal antibody (STJ24413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Lamin B1 antibody (STJ24413). Western blot was performed from the immunoprecipitate using Lamin B1 antibody (STJ24413) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Lamin B1 antibody (STJ24413). Western blot was performed from the immunoprecipitate using Lamin B1 (STJ24413) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Lamin B1 rabbit polyclonal antibody antibody (STJ24413) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-LMNB1 antibody (397-588) (STJ24413)

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STJ24413

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Lamin B1 (397-588) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB1
Gene ID: 4001
Uniprot ID: LMNB1_HUMAN
Immunogen Region: 397-588
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 397-588 of human Lamin B1 (NP_005564.1).
Immunogen Sequence: RVTVSRASSSRSVRTTRGKR KRVDVEESEASSSVSISHSA SATGNVCIEEIDVDGKFIRL KNTSEQDQPMGGWEMIRKIG DTSVSYKYTSRYVLKAGQTV TIWAANAGVTASPPTDLIWK NQNSWGTGEDVKVILKNSQG EEVAQRSTVFKTTIPEEEEE EEEAAGVVVEEELFHQQGTP RASNRSCAIM
Post Translational Modifications B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Protein Name Lamin-B1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2559584
Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Reactome: R-HSA-352238
Reactome: R-HSA-4419969
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9013405
Reactome: R-HSA-9035034
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B1 antibody
Anti-LMNB1 antibody
Anti-LMN2 antibody
Anti-LMNB antibody

Information sourced from Uniprot.org

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