Western blot analysis of lysates from SH-SY5Y cells, using Cytokeratin 9 (KRT9) Rabbit mAb (STJ11102508) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-KRT9 antibody (254-412) [S8MR] (STJ11102508)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102508
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General Information

Short DescriptionRabbit monoclonal Cytokeratin 9 (254-412) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS8MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolKRT9
Gene ID3857
Uniprot IDK1C9_HUMAN
Immunogen Region254-412
Immunogen SequenceDADINGLRQVLDNLTMEKSD LEMQYETLQEELMALKKNHK EEMSQLTGQNSGDVNVEINV APGKDLTKTLNDMRQEYEQL IAKNRKDIENQYETQITQIE HEVSSSGQEVQSSAKEVTQL RHGVQELEIELQSQLSKKAA LEKSLEDTKNRYCGQLQMI
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 254-412 of human Cytokeratin 9 (KRT9) (P35527).

Additional Info

Tissue Specificity Expressed in the terminally differentiated epidermis of palms and soles.
Function May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Protein Name Keratin - Type I Cytoskeletal 9
Cytokeratin-9
Ck-9
Keratin-9
K9
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 9 antibody
Anti-Cytokeratin-9 antibody
Anti-Ck-9 antibody
Anti-Keratin-9 antibody
Anti-K9 antibody
Anti-KRT9 antibody

Information sourced from Uniprot.org

Citations

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