• Western blot analysis of various lysates, using KMT2B antibody (STJ11101537) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-KMT2B antibody (1370-1570) (STJ11101537)

SKU:
STJ11101537

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KMT2B (1370-1570) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KMT2B
Gene ID: 9757
Uniprot ID: KMT2B_HUMAN
Immunogen Region: 1370-1570
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1370-1570 of human KMT2B (NP_055542.1).
Immunogen Sequence: GLSDEDYEILSGLPDSVLYT CGPCAGAAQPRWREALSGAL QGGLRQVLQGLLSSKVVGPL LLCTQCGPDGKQLHPGPCGL QAVSQRFEDGHYKSVHSFME DMVGILMRHSEEGETPDRRA GGQMKGLLLKLLESAFGWFD AHDPKYWRRSTRLPNGVLPN AVLPPSLDHVYAQWRQQEPE TPESGQPPGDPSAAFQGKDP A
Tissue Specificity Widely expressed. Highest levels in testis. Also found in brain with higher expression in the cerebellum than in any other region, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.
Function Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place. Likely plays a redundant role with KMT2C in enriching H3K4me1 marks on primed and active enhancer elements. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
Protein Name Histone-Lysine N-Methyltransferase 2b
Lysine N-Methyltransferase 2b
Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 4
Trithorax Homolog 2
Ww Domain-Binding Protein 7
Wbp-7
Database Links Reactome: R-HSA-3214841
Reactome: R-HSA-8936459
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase 2b antibody
Anti-Lysine N-Methyltransferase 2b antibody
Anti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 4 antibody
Anti-Trithorax Homolog 2 antibody
Anti-Ww Domain-Binding Protein 7 antibody
Anti-Wbp-7 antibody
Anti-KMT2B antibody
Anti-HRX2 antibody
Anti-KIAA0304 antibody
Anti-MLL2 antibody
Anti-MLL4 antibody
Anti-TRX2 antibody
Anti-WBP7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance