• Immunofluorescence analysis of U-2 OS cells using KLHL8 Rabbit polyclonal antibody (STJ117116) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using KLHL8 Rabbit polyclonal antibody (STJ117116) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using KLHL8 Rabbit polyclonal antibody (STJ117116) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using KLHL8 Rabbit polyclonal antibody (STJ117116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KLHL8 antibody (271-620) (STJ117116)

SKU:
STJ117116

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLHL8 (271-620) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLHL8
Gene ID: 57563
Uniprot ID: KLHL8_HUMAN
Immunogen Region: 271-620
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 271-620 of human KLHL8 (NP_065854.3).
Immunogen Sequence: AKEQIVKQNLKCRDLLDEAR NYHLHLSSRAVPDFEYSIRT TPRKHTAGVLFCVGGRGGSG DPFRSIECYSINKNSWFFGP EMNSRRRHVGVISVEGKVYA VGGHDGNEHLGSMEMFDPLT NKWMMKASMNTKRRGIALAS LGGPIYAIGGLDDNTCFNDV ERYDIESDQWSTVAPMNTPR GGVGSVALVNHVYAVGGNDG MASLSSVERYDPHLDKWIEV KEMGQRRAGNGVSKLHGCL
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase complex mediates ubiquitination and degradation of RAPSN.
Protein Name Kelch-Like Protein 8
Alternative Antibody Names Anti-Kelch-Like Protein 8 antibody
Anti-KLHL8 antibody
Anti-KIAA1378 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance