Anti-KLF4 antibody (STJA0037968)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human KLF4
General Information
| Short Description | Rabbit monoclonal anti-KLF4 for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | IHC 1:100-1:200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | KLF4 |
| Gene ID | 9314 |
| Uniprot ID | KLF4_HUMAN |
| Immunogen | Recombinant protein of human KLF4 |
Additional Info
| Post Translational Modifications | Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis. Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6.deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis. |
| Function | Transcription factor.can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. |
| Protein Name | Krueppel-Like Factor 4Epithelial Zinc Finger Protein EzfGut-Enriched Krueppel-Like Factor |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-422085Reactome: R-HSA-452723Reactome: R-HSA-9617828 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Krueppel-Like Factor 4 antibodyAnti-Epithelial Zinc Finger Protein Ezf antibodyAnti-Gut-Enriched Krueppel-Like Factor antibodyAnti-KLF4 antibodyAnti-EZF antibodyAnti-GKLF antibody |
Information sourced from Uniprot.org