Western blot analysis of various lysates using KLF10 Rabbit polyclonal antibody (STJ115146) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KLF10 antibody (161-365) (STJ115146)

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STJ115146
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF10
Gene ID: 7071
Uniprot ID: KLF10_HUMAN
Immunogen Region: 161-365
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 161-365 of human KLF10 (NP_001027453.1).
Immunogen Sequence: MKAASILNYQNNSFRRRTHL NVEAARKNIPCAAVSPNRSK CERNTVADVDEKASAALYDF SVPSSETVICRSQPAPVSPQ QKSVLVSPPAVSAGGVPPMP VICQMVPLPANNPVVTTVVP STPPSQPPAVCPPVVFMGTQ VPKGAVMFVVPQPVVQSSKP PVVSPNGTRLSPIAPAPGFS PSAAKVTPQIDSSRIRSHIC SHPGC
Post Translational Modifications Ubiquitinated.mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Function Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock.binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis. May play a role in the cell cycle regulation.
Protein Name Krueppel-Like Factor 10
Egr-Alpha
Transforming Growth Factor-Beta-Inducible Early Growth Response Protein 1
Tgfb-Inducible Early Growth Response Protein 1
Tieg-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Krueppel-Like Factor 10 antibody
Anti-Egr-Alpha antibody
Anti-Transforming Growth Factor-Beta-Inducible Early Growth Response Protein 1 antibody
Anti-Tgfb-Inducible Early Growth Response Protein 1 antibody
Anti-Tieg-1 antibody
Anti-KLF10 antibody
Anti-TIEG antibody
Anti-TIEG1 antibody

Information sourced from Uniprot.org

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