Anti-KLF1 antibody (16-34) (STJ112595)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KLF1 (16-34) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KLF1 |
| Gene ID | 10661 |
| Uniprot ID | KLF1_HUMAN |
| Immunogen Region | 16-34 |
| Immunogen Sequence | MATAETALPSISTLTALGPF PDTQDDFLKWWRSEEAQDMG PGPPDPTEPPLHVKSEDQPG EEEDDERGADATWDLDLLLT NFSGPEPGGAPQTCALAPSE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLF1 (NP_006554.1). |
Additional Info
| Tissue Specificity | Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines. |
| Post Translational Modifications | Acetylated.can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity. Sumoylated.sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex. Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity. |
| Function | Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. |
| Protein Name | Krueppel-Like Factor 1Erythroid Krueppel-Like Transcription FactorEklf |
| Database Links | |
| Cellular Localisation | NucleusColocalizes With Sumo1 In Nuclear Speckles |
| Alternative Antibody Names | Anti-Krueppel-Like Factor 1 antibodyAnti-Erythroid Krueppel-Like Transcription Factor antibodyAnti-Eklf antibodyAnti-KLF1 antibodyAnti-EKLF antibody |
Information sourced from Uniprot.org