• Western blot analysis of extracts of various cell lines, using KIR3DS1 antibody (STJ111977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using KIR3DS1 antibody (STJ111977) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using KIR3DS1 antibody (STJ111977) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver using KIR3DS1 antibody (STJ111977) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human mammary cancer using KIR3DS1 antibody (STJ111977) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using KIR3DS1 antibody (STJ111977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-KIR3DS1 antibody (80-340) (STJ111977)

SKU:
STJ111977

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIR3DS1 (80-340) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIR3DS1
Gene ID: 3813
Uniprot ID: KI3S1_HUMAN
Immunogen Region: 80-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human KIR3DS1 (NP_001077008.1).
Immunogen Sequence: FNMSPVTTAHAGNYTCRGSH PHSPTGWSAPSNPMVIMVTG NHRKPSLLAHPGPLVKSGER VILQCWSDIMFEHFFLHKEW ISKDPSRLVGQIHDGVSKAN FSIGSMMRALAGTYRCYGSV THTPYQLSAPSDPLDIVVTG LYEKPSLSAQPGPKVQAGES VTLSCSSRSSYDMYHLSREG GAHERRLPAVRKVNRTFQAD FPLGPATHGGTYRCFGSFRH SPYEWSDPSDPLLVSVTGN
Tissue Specificity Expressed in NK and T-cell lines but not in B-lymphoblastoid cell lines or in a colon carcinoma cell line.
Function Receptor on natural killer (NK) cells for MHC class I molecules. Upon interaction with peptide-free HLA-F open conformer, triggers NK cell degranulation and anti-viral cytokine production.
Protein Name Killer Cell Immunoglobulin-Like Receptor 3ds1
Natural Killer-Associated Transcript 10
Nkat-10
Database Links Reactome: R-HSA-2172127
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Killer Cell Immunoglobulin-Like Receptor 3ds1 antibody
Anti-Natural Killer-Associated Transcript 10 antibody
Anti-Nkat-10 antibody
Anti-KIR3DS1 antibody
Anti-NKAT10 antibody

Information sourced from Uniprot.org

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