• Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit monoclonal antibody (STJ11102673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit monoclonal antibody (STJ11102673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using KIFC1 Rabbit monoclonal antibody (STJ11102673) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using KIFC1 antibody (STJ11102673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-KIFC1 antibody (1-100) [S3MR] (STJ11102673)

SKU:
STJ11102673

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-KIFC1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIFC1
Gene ID: 3833
Uniprot ID: KIFC1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KIFC1 (Q9BW19).
Immunogen Sequence: MDPQRSPLLEVKGNIELKRP LIKAPSQLPLSGSRLKRRPD QMEDGLEPEKKRTRGLGATT KITTSHPRVPSLTTVPQTQG QTTAQKVSKKTGPRCSTAIA
Function Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Regulates cilium formation and structure.
Protein Name Kinesin-Like Protein Kifc1
Kinesin-Like Protein 2
Kinesin-Related Protein Hset
Database Links Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Early Endosome
Associated With Nucleus During Interphase
Centrosomes In Early And Spindle In Later Mitosis
Alternative Antibody Names Anti-Kinesin-Like Protein Kifc1 antibody
Anti-Kinesin-Like Protein 2 antibody
Anti-Kinesin-Related Protein Hset antibody
Anti-KIFC1 antibody
Anti-HSET antibody
Anti-KNSL2 antibody

Information sourced from Uniprot.org

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