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Anti-KIF3A antibody (600-699) [S4MR] (STJ11103614)

Immunofluorescence analysis of NIH-3T3 cells using KIF3A Rabbit monoclonal antibody (STJ11103614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded rat testis using KIF3A Rabbit monoclonal antibody (STJ11103614) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Western blot analysis of extracts of various cell lines, using KIF3A antibody (STJ11103614) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KIF3A antibody (600-699) [S4MR] (STJ11103614)

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STJ11103614

£212.50 - £501.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-KIF3A (600-699) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S4MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:100-1:500 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	KIF3A
Gene ID:	11127
Uniprot ID:	KIF3A_HUMAN
Immunogen Region:	600-699
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 600-699 of human KIF3A (Q9Y496).
Immunogen Sequence:	YQEMIENYVHWNEDIGEWQL KCVAYTGNNMRKQTPVPDKK EKDPFEVDLSHVYLAYTEES LRQSLMKLERPRTSKGKARP KTGRRKRSAKPETVIDSLLQ

Function	Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.
Protein Name	Kinesin-Like Protein Kif3a Microtubule Plus End-Directed Kinesin Motor 3a
Database Links	Reactome: R-HSA-1445148 Reactome: R-HSA-2132295 Reactome: R-HSA-5610787 Reactome: R-HSA-5620924 Reactome: R-HSA-5635838 Reactome: R-HSA-6811434 Reactome: R-HSA-983189
Cellular Localisation	Cytoplasm Cytoskeleton Cell Projection Cilium Microtubule Organizing Center Centrosome Centriole Localizes To The Subdistal Appendage Region Of The Centriole
Alternative Antibody Names	Anti-Kinesin-Like Protein Kif3a antibody Anti-Microtubule Plus End-Directed Kinesin Motor 3a antibody Anti-KIF3A antibody Anti-KIF3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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