• Western blot analysis of lysates from SKOV3 cells, using KIF1B Rabbit polyclonal antibody (STJ28721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KIF1B antibody (1501-1770) (STJ28721)

SKU:
STJ28721

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF1B
Gene ID: 23095
Uniprot ID: KIF1B_HUMAN
Immunogen Region: 1501-1770
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1501-1770 of human KIF1B (NP_055889.2).
Immunogen Sequence: SQISTTTFESAITPSESSGY DSGDIESLVDREKELATKCL QLLTHTFNREFSQVHGSVSD CKLSDISPIGRDPSESSFSS ATLTPSSTCPSLVDSRSNSL DQKTPEANSRASSPCPEFEQ FQIVPAVETPYLARAGKNEF LNLVPDIEEIRPSSVVSKKG YLHFKEPLYSNWAKHFVVVR RPYVFIYNSDKDPVERGIIN LSTAQVEYSEDQQAMVKTPN TFAVCTKHRGVLLQALNDK
Tissue Specificity Isoform 3 is abundant in the skeletal muscle. It is also expressed in fetal brain, lung and kidney, and adult heart, placenta, testis, ovary and small intestine. Isoform 2 is abundant in the brain and also expressed in fetal heart, lung, liver and kidney, and adult skeletal muscle, placenta, liver, kidney, heart, spleen, thymus, prostate, testis, ovary, small intestine, colon and pancreas.
Function Has a plus-end-directed microtubule motor activity and functions as a motor for transport of vesicles and organelles along microtubules. Isoform 2: Has a plus-end-directed microtubule motor activity and functions as a motor for anterograde synaptic vesicle transport along axonal microtubules from the cell body to the presynapse in neuronal cells. Functions as a downstream effector in a developmental apoptotic pathway that is activated when nerve growth factor (NGF) becomes limiting for neuronal progenitor cells. Isoform 3: Has a plus-end-directed microtubule motor activity and functions as a motor for anterograde transport of mitochondria.
Protein Name Kinesin-Like Protein Kif1b
Klp
Database Links Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Has A Plus-End-Directed Microtubule Motor Activity And Therefore Associates With Microtubules
Isoform 2: Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Associates With Synaptic Vesicles And Mediates Their Anterograde Transport Along Axonal Microtubules
Isoform 3: Mitochondrion
Associates With Mitochondria And Mediates Their Movement Along Microtubules
Alternative Antibody Names Anti-Kinesin-Like Protein Kif1b antibody
Anti-Klp antibody
Anti-KIF1B antibody
Anti-KIAA0591 antibody
Anti-KIAA1448 antibody

Information sourced from Uniprot.org

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