| Host: |
Mouse |
| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CXCL8 is suitable for use in ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
1501 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
50 mM Na-citrate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative |
| Purification: |
Purity accurate to greater than or equal to 95% |
| Concentration: |
5.0 mg/ml (+/-10 %) |
| Storage Instruction: |
18 months from manufacturing at 2°C. Temperature analysis:-70 °C, 21 days N/D-20 °C, 21 days OK +4 °C, 21 days OK +30 °C, 21 OK +35 °C, 21 days OK +45 °C, 7 days OK. Shelf life stability testing at 2–8 °C in the product buffer. |
| Association Rate Constant: |
8.9 x 105 1/Ms |
| Determination Method: |
SPR analysis (ProteOn XPR36) |
| Immunoreactivity: |
80–120 % compared to the reference sample in an FIA test |
| Post Translational Modifications | Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. (Microbial infection) Cleaved by group A Streptococcus protease SpyCEP.leading to impaired neutrophil endothelial transmigration and thus increased virulence. |
| Function | Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. |
| Protein Name | Interleukin-8Il-8C-X-C Motif Chemokine 8ChemokineC-X-C Motif Ligand 8EmoctakinGranulocyte Chemotactic Protein 1Gcp-1Monocyte-Derived Neutrophil Chemotactic FactorMdncfMonocyte-Derived Neutrophil-Activating PeptideMonapNeutrophil-Activating Protein 1Nap-1Protein 3-10cT-Cell Chemotactic Factor Cleaved Into - Mdncf-AGcp/Il-8 Protein IvIl8/Nap1 Form I - Interleukin-8(Ala-Il-877Gcp/Il-8 Protein IiIl-8(1-77Il8/Nap1 Form IiMdncf-B - Il-8(5-77 - Il-8(6-77(Ser-Il-872Gcp/Il-8 Protein IIl8/Nap1 Form IiiLymphocyte-Derived Neutrophil-Activating FactorLynapMdncf-CNeutrophil-Activating FactorNaf - Il-8(7-77Gcp/Il-8 Protein VIl8/Nap1 Form Iv - Il-8(8-77Gcp/Il-8 Protein ViIl8/Nap1 Form V - Il-8(9-77Gcp/Il-8 Protein IiiIl8/Nap1 Form Vi |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-375276Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-418594Reactome: R-HSA-6783783Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-8 antibodyAnti-Il-8 antibodyAnti-C-X-C Motif Chemokine 8 antibodyAnti-Chemokine antibodyAnti-C-X-C Motif Ligand 8 antibodyAnti-Emoctakin antibodyAnti-Granulocyte Chemotactic Protein 1 antibodyAnti-Gcp-1 antibodyAnti-Monocyte-Derived Neutrophil Chemotactic Factor antibodyAnti-Mdncf antibodyAnti-Monocyte-Derived Neutrophil-Activating Peptide antibodyAnti-Monap antibodyAnti-Neutrophil-Activating Protein 1 antibodyAnti-Nap-1 antibodyAnti-Protein 3-10c antibodyAnti-T-Cell Chemotactic Factor Cleaved Into - Mdncf-A antibodyAnti-Gcp/Il-8 Protein Iv antibodyAnti-Il8/Nap1 Form I - Interleukin-8 antibodyAnti-(Ala-Il-877 antibodyAnti-Gcp/Il-8 Protein Ii antibodyAnti-Il-8(1-77 antibodyAnti-Il8/Nap1 Form Ii antibodyAnti-Mdncf-B - Il-8(5-77 - Il-8(6-77 antibodyAnti-(Ser-Il-872 antibodyAnti-Gcp/Il-8 Protein I antibodyAnti-Il8/Nap1 Form Iii antibodyAnti-Lymphocyte-Derived Neutrophil-Activating Factor antibodyAnti-Lynap antibodyAnti-Mdncf-C antibodyAnti-Neutrophil-Activating Factor antibodyAnti-Naf - Il-8(7-77 antibodyAnti-Gcp/Il-8 Protein V antibodyAnti-Il8/Nap1 Form Iv - Il-8(8-77 antibodyAnti-Gcp/Il-8 Protein Vi antibodyAnti-Il8/Nap1 Form V - Il-8(9-77 antibodyAnti-Gcp/Il-8 Protein Iii antibodyAnti-Il8/Nap1 Form Vi antibodyAnti-CXCL8 antibodyAnti-IL8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
