• Immunohistochemistry analysis of KIAA0196 in paraffin-embedded mouse spinal cord using KIAA0196 Rabbit polyclonal antibody (STJ11103249) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KIAA0196 in paraffin-embedded rat brain using KIAA0196 Rabbit polyclonal antibody (STJ11103249) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse thymus, using KIAA0196 Rabbit polyclonal antibody (STJ11103249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-KIAA0196 antibody (380-530) (STJ11103249)

SKU:
STJ11103249

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIAA0196 (380-530) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WASHC5
Gene ID: 9897
Uniprot ID: WASC5_HUMAN
Immunogen Region: 380-530
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-530 of human KIAA0196 (NP_055661.3).
Immunogen Sequence: TADSACDPNNKRLRQIKDQI LTDSRYNPRILFQLLLDTAQ FEFILKEMFKQMLSEKQTKW EHYKKEGSERMTELADVFSG VKPLTRVEKNENLQAWFREI SKQILSLNYDDSTAAGRKTV QLIQALEEVQEFHQLESNLQ VCQFLADTRKF
Tissue Specificity Expressed ubiquitously.
Function Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. May be involved in axonal outgrowth. Involved in cellular localization of ADRB2. Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7.
Protein Name Wash Complex Subunit 5
Strumpellin
Wash Complex Subunit Strumpellin
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum
Early Endosome
Colocalizes With Syp/Synaptophysin In The External Molecular Layer Of The Dentate Gyrus And In Motoneurons Of The Ventral Horn Of Spinal Cord
Alternative Antibody Names Anti-Wash Complex Subunit 5 antibody
Anti-Strumpellin antibody
Anti-Wash Complex Subunit Strumpellin antibody
Anti-WASHC5 antibody
Anti-KIAA0196 antibody

Information sourced from Uniprot.org

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