![Anti-Keratin 5+14 antibody [EBS-IF-001] (STJ16101016)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149732/302473/STJ16101016_1__65762.1646940654.jpg?c=1 "Anti-Keratin 5+14 antibody [EBS-IF-001] (STJ16101016)")
![Anti-Keratin 5+14 antibody [EBS-IF-001] (STJ16101016)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149732/302473/STJ16101016_1__65762.1646940654.jpg?c=1 "Anti-Keratin 5+14 antibody [EBS-IF-001] (STJ16101016)")
Anti-Keratin 5+14 antibody [EBS-IF-001] (STJ16101016)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenA hybrid (BALB/c x B6) mouse was immunized with a cytokeratin-enriched extract of feline tongue epithelium. Fusion partner: Sp2/0.
General Information
| Short Description | Mouse monoclonal anti-Keratin 5+14 for use in FC, IF and IHC in Cat and Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC |
| Host | Mouse |
| Reactivity | Cat/Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | EBS-IF-001 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate anti |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Immunogen | A hybrid (BALB/c x B6) mouse was immunized with a cytokeratin-enriched extract of feline tongue epithelium. Fusion partner: Sp2/0. |
Additional Info
| Background | EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells. |
Information sourced from Uniprot.org