• Western blot analysis of extracts of various cell lines, using KDM6A antibody (STJ110458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Kdm6a antibody (STJ110458) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human breast cancer using Kdm6a antibody (STJ110458) at dilution of 1:200 (40x lens).
  • Western blot analysis of various lysates, using KDM6A antibody (STJ110458) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Kdm6a antibody (550-700) (STJ110458)

SKU:
STJ110458

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KDM6A (550-700) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Kdm6a
Gene ID: 22289
Uniprot ID: KDM6A_MOUSE
Immunogen Region: 550-700
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 550-700 of mouse KDM6A (NP_033509.1).
Immunogen Sequence: MPSVSQPGVHTACPRQTLAN GPFSAGHVPCSTSRTLGSTD TVLIGNNHVTGSGSNGNVPY LQRNAPTLPHNRTNLTSSTE EPWKNQLSNSTQGLHKGPSS HLAGPNGERPLSSTGPSQHL QAAGSGIQNQNGHPTLPSNS VTQGAALNHLS
Tissue Specificity Expressed in brain, heart and spleen.
Function Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A. Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression.
Protein Name Lysine-Specific Demethylase 6a
Histone Demethylase Utx
Ubiquitously Transcribed Tpr Protein On The X Chromosome
Ubiquitously Transcribed X Chromosome Tetratricopeptide Repeat Protein
Histone H3-Trimethyl-L-Lysine(27 Demethylase 6a
Database Links Reactome: R-MMU-3214842
Reactome: -MMU-9772755
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Lysine-Specific Demethylase 6a antibody
Anti-Histone Demethylase Utx antibody
Anti-Ubiquitously Transcribed Tpr Protein On The X Chromosome antibody
Anti-Ubiquitously Transcribed X Chromosome Tetratricopeptide Repeat Protein antibody
Anti-Histone H3-Trimethyl-L-Lysine(27 Demethylase 6a antibody
Anti-Kdm6a antibody
Anti-Utx antibody

Information sourced from Uniprot.org

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