Anti-KCNN4 antibody (177-227 aa) (STJ197363)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide derived from KCNN4 (SK4) at the amino acid range 177-227
General Information
| Short Description | Rabbit polyclonal anti-Intermediate conductance calcium-activated potassium channel protein 4 (177-227 aa) for use in WB, IHC and IF in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000IHC 1:50-100IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KCNN4 |
| Gene ID | 3783 |
| Uniprot ID | KCNN4_HUMAN |
| Immunogen | Synthetic peptide derived from KCNN4 (SK4) at the amino acid range 177-227 |
| Immunogen Region | 177-227 aa |
| Specificity | KCNN4 (SK4) protein (A247) detects endogenous levels of KCNN4 (SK4) |
Additional Info
| Post Translational Modifications | Phosphorylation at His-358 by NDKB activates the intermediate conductance calcium-activated potassium channel activity, and conversely it's dephosphorylation by PHPT1 inhibits this activity. |
| Function | Intermediate conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening. The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 25 picosiemens. Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV. Controls calcium influx during vascular contractility by being responsible of membrane hyperpolarization induced by vasoactive factors in proliferative vascular smooth muscle cell types. Following calcium influx, the consecutive activation of KCNN4 channel leads to a hyperpolarization of the cell membrane potential and hence an increase of the electrical driving force for further calcium influx promoting sustained calcium entry in response to stimulation with chemotactic peptides. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis through activation by PI(3)P. |
| Protein Name | Intermediate Conductance Calcium-Activated Potassium Channel Protein 4Skca 4Skca4Hsk4Gardos ChannelIkca1Hik1Kca3.1Putative Gardos ChannelHkca4 |
| Database Links | Reactome: R-HSA-1296052 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionRuffle MembraneTargeted To Membrane Ruffles After Egf Stimulation |
| Alternative Antibody Names | Anti-Intermediate Conductance Calcium-Activated Potassium Channel Protein 4 antibodyAnti-Skca 4 antibodyAnti-Skca4 antibodyAnti-Hsk4 antibodyAnti-Gardos Channel antibodyAnti-Ikca1 antibodyAnti-Hik1 antibodyAnti-Kca3.1 antibodyAnti-Putative Gardos Channel antibodyAnti-Hkca4 antibodyAnti-KCNN4 antibodyAnti-IK1 antibodyAnti-IKCA1 antibodyAnti-KCA4 antibodyAnti-SK4 antibody |
Information sourced from Uniprot.org