• Western blot analysis of various lysates, using JMJD4 antibody (STJ110299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of PC-12 cells using JMJD4 rabbit polyclonal antibody (STJ110299) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-JMJD4 antibody (1-463) (STJ110299)

SKU:
STJ110299

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JMJD4
Gene ID: 65094
Uniprot ID: JMJD4_HUMAN
Immunogen Region: 1-463
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human JMJD4 (NP_075383.2).
Immunogen Sequence: MRAGPEPQALAGQKRGALRL LVPRLVLTVSAPAEVRRRVL RPVLSWMDRETRALADSHFR GLGVDVPGVGQAPGRVAFVS EPGAFSYADFVRGFLLPNLP CVFSSAFTQGWGSRRRWVTP AGRPDFDHLLRTYGDVVVPV ANCGVQEYNSNPKEHMTLRD YITYWKEYIQAGYSSPRGCL YLKDWHLCRDFPVEDVFTLP VYFSSDWLNEFWDALDVDDY RFVYAGPAGSWSPFHADIF
Function Catalyzes the 2-oxoglutarate and iron-dependent C4-lysyl hydroxylation of ETF1 at 'Lys-63' thereby promoting the translational termination efficiency of ETF1.
Protein Name 2-Oxoglutarate And Iron-Dependent Oxygenase Jmjd4
Jmjc Domain-Containing Protein 4
Jumonji Domain-Containing Protein 4
Lysyl-Hydroxylase Jmjd4
Database Links Reactome: R-HSA-9629569
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-2-Oxoglutarate And Iron-Dependent Oxygenase Jmjd4 antibody
Anti-Jmjc Domain-Containing Protein 4 antibody
Anti-Jumonji Domain-Containing Protein 4 antibody
Anti-Lysyl-Hydroxylase Jmjd4 antibody
Anti-JMJD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance