• Western blot analysis of lysates from RAW 264. 7 cells using CD11b Rabbit polyclonal antibody (STJ11105585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:0. 5s.

Anti-ITGAM antibody (1-100) (STJ11105585)

SKU:
STJ11105585

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Integrin Alpha-M (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGAM
Gene ID: 3684
Uniprot ID: ITAM_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD11b (NP_000623.2).
Immunogen Sequence: MALRVLLLTALTLCHGFNLD TENAMTFQENARGFGQSVVQ LQGSRVVVGAPQEIVAANQR GSLYQCDYSTGSCEPIRLQV PVEAVNMSLGLSLAATTSPP
Tissue Specificity Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
Function Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.
Protein Name Integrin Alpha-M
Cd11 Antigen-Like Family Member B
Cr-3 Alpha Chain
Cell Surface Glycoprotein Mac-1 Subunit Alpha
Leukocyte Adhesion Receptor Mo1
Neutrophil Adherence Receptor
Cd Antigen Cd11b
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Alternative Antibody Names Anti-Integrin Alpha-M antibody
Anti-Cd11 Antigen-Like Family Member B antibody
Anti-Cr-3 Alpha Chain antibody
Anti-Cell Surface Glycoprotein Mac-1 Subunit Alpha antibody
Anti-Leukocyte Adhesion Receptor Mo1 antibody
Anti-Neutrophil Adherence Receptor antibody
Anti-Cd Antigen Cd11b antibody
Anti-ITGAM antibody
Anti-CD11B antibody
Anti-CR3A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance