Anti-IST1 antibody (1-335) (STJ116357)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IST1 (1-335) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IST1 |
| Gene ID | 9798 |
| Uniprot ID | IST1_HUMAN |
| Immunogen Region | 1-335 |
| Immunogen Sequence | MLGSGFKAERLRVNLRLVIN RLKLLEKKKTELAQKARKEI ADYLAAGKDERARIRVEHII REDYLVEAMEILELYCDLLL ARFGLIQSMKELDSGLAESV STLIWAAPRLQSEVAELKIV ADQLCAKYSKEYGKLCRTNQ IGTVNDRLMHKLSVEAPPKI LVERYLIEIAKNYNVPYEPD SVVMAEAPPGVETDLIDVGF TDDVKKGGPGRGGSGGFTAP VGGPDGTVPMPMPMPMPMP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human IST1 (NP_001257906.1). |
Additional Info
| Function | ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST. |
| Protein Name | Ist1 HomologHist1Charged Multivesicular Body Protein 8Chmp8Putative Mapk-Activating Protein Pm28 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-9668328 |
| Cellular Localisation | Cytoplasmic VesicleCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeMidbodyNucleus EnvelopeLocalizes To Centrosome And Midbody Of Dividing CellsColocalized With Spart To The Ends Of Flemming Bodies During CytokinesisLocalizes To The Reforming Nuclear Envelope On Chromatin Disks During Late Anaphase |
| Alternative Antibody Names | Anti-Ist1 Homolog antibodyAnti-Hist1 antibodyAnti-Charged Multivesicular Body Protein 8 antibodyAnti-Chmp8 antibodyAnti-Putative Mapk-Activating Protein Pm28 antibodyAnti-IST1 antibodyAnti-KIAA0174 antibody |
Information sourced from Uniprot.org