• Western blot analysis of extracts of various cell lines, using IRAK3 antibody (STJ27420) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-IRAK3 antibody (STJ27420)

SKU:
STJ27420
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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against IRAK3.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: IRAK3_HUMAN
Gene ID: 11213
Gene Symbol: IRAK3
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 367-596 of human IRAK3 (NP_009130.2).
Background This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.
Tissue Specificity Expressed predominantly in peripheral blood lymphocytes.
Function Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
Database Links Reactome: R-HSA-166058
Protein Name Interleukin-1 Receptor-Associated Kinase 3
Irak-3
Il-1 Receptor-Associated Kinase M
Irak-M
Alternative Names Anti-Interleukin-1 Receptor-Associated Kinase 3 antibody
Anti-Irak-3 antibody
Anti-Il-1 Receptor-Associated Kinase M antibody
Anti-Irak-M antibody
Anti-IRAK3 antibody

Information sourced from uniprot.org

6 months standard