| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IQGAP1 |
| Gene ID: | 8826 |
| Uniprot ID: | IQGA1_HUMAN |
| Immunogen Region: | 500-750 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_003861.1). |
| Immunogen Sequence: | ELMKLKAQAHAENNEFITWN DIQACVDHVNLVVQEEHERI LAIGLINEALDEGDAQKTLQ ALQIPAAKLEGVLAEVAQHY QDTLIRAKREKAQEIQDESA VLWLDEIQGGIWQSNKDTQE AQKFALGIFAINEAVESGDV GKTLSALRSPDVGLYGVIPE CGETYHSDLAEAKKKKLAVG DNNSKWVKHWVKGGYYYYHN LETQEGGWDEPPNFVQNSMQ LSREEIQSSISGVTAAYNR |
| Tissue Specificity | Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas. |
| Post Translational Modifications | Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. |
| Function | Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest. |
| Protein Name | Ras Gtpase-Activating-Like Protein Iqgap1P195 |
| Database Links | Reactome: R-HSA-373753Reactome: R-HSA-381676Reactome: R-HSA-5626467Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013406Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell MembraneNucleusCytoplasmCell CortexApical Cell MembraneBasolateral Cell MembraneSubcellular Distribution Is Regulated By The Cell CycleNuclear Levels Increase At G1/S Phase |
| Alternative Antibody Names | Anti-Ras Gtpase-Activating-Like Protein Iqgap1 antibodyAnti-P195 antibodyAnti-IQGAP1 antibodyAnti-KIAA0051 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
