| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:100 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IQGAP1 |
| Gene ID: | 8826 |
| Uniprot ID: | IQGA1_HUMAN |
| Immunogen Region: | 500-750 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_003861.1). |
| Immunogen Sequence: | ELMKLKAQAHAENNEFITWN DIQACVDHVNLVVQEEHERI LAIGLINEALDEGDAQKTLQ ALQIPAAKLEGVLAEVAQHY QDTLIRAKREKAQEIQDESA VLWLDEIQGGIWQSNKDTQE AQKFALGIFAINEAVESGDV GKTLSALRSPDVGLYGVIPE CGETYHSDLAEAKKKKLAVG DNNSKWVKHWVKGGYYYYHN LETQEGGWDEPPNFVQNSMQ LSREEIQSSISGVTAAYNR |
| Tissue Specificity | Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas. |
| Post Translational Modifications | Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. |
| Function | Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest. |
| Protein Name | Ras Gtpase-Activating-Like Protein Iqgap1 P195 |
| Database Links | Reactome: R-HSA-373753 Reactome: R-HSA-381676 Reactome: R-HSA-5626467 Reactome: R-HSA-5674135 Reactome: R-HSA-6798695 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-8980692 Reactome: R-HSA-9013106 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013406 Reactome: R-HSA-9013420 Reactome: R-HSA-9013424 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell Membrane Nucleus Cytoplasm Cell Cortex Apical Cell Membrane Basolateral Cell Membrane Subcellular Distribution Is Regulated By The Cell Cycle Nuclear Levels Increase At G1/S Phase |
| Alternative Antibody Names | Anti-Ras Gtpase-Activating-Like Protein Iqgap1 antibody Anti-P195 antibody Anti-IQGAP1 antibody Anti-KIAA0051 antibody |
Information sourced from Uniprot.org