• Immunofluorescence analysis of mouse spleen using SHIP11 Rabbit monoclonal antibody (STJ11101903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human spleen using SHIP11 Rabbit monoclonal antibody (STJ11101903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen using SHIP11 Rabbit monoclonal antibody (STJ11101903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using SHIP11 Rabbit monoclonal antibody (STJ11101903) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SHIP11 Rabbit monoclonal antibody (STJ11101903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-INPP5D antibody (1084-1178) [S3MR] (STJ11101903)

SKU:
STJ11101903

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SHIP (1084-1178) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INPP5D
Gene ID: 3635
Uniprot ID: SHIP1_HUMAN
Immunogen Region: 1084-1178
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1084-1178 of human SHIP11 (Q92835).
Immunogen Sequence: RSFTCSSSAEGRAAGGDKSQ GKPKTPVSSQAPVPAKRPIK PSRSEINQQTPPTPTPRPPL PVKSPAVLHLQHSKGRDYRD NTELPHHGKHRPEEG
Tissue Specificity Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
Post Translational Modifications Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.
Function Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.
Protein Name Phosphatidylinositol 3 -4 -5-Trisphosphate 5-Phosphatase 1
Inositol Polyphosphate-5-Phosphatase D
Inositol Polyphosphate-5-Phosphatase Of 145 Kda
Sip-145
Phosphatidylinositol 4 -5-Bisphosphate 5-Phosphatase
Sh2 Domain-Containing Inositol 5'-Phosphatase 1
Sh2 Domain-Containing Inositol Phosphatase 1
Ship-1
P150ship
Hp51cn
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1855204
Reactome: R-HSA-202424
Reactome: R-HSA-210990
Reactome: R-HSA-912526
Reactome: R-HSA-9680350
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Membrane Raft
Cytoskeleton
Membrane
Translocates To The Plasma Membrane When Activated
Translocation Is Probably Due To Different Mechanisms Depending On The Stimulus And Cell Type
Translocates From The Cytoplasm To Membrane Ruffles In A Fcgr3/Cd16-Dependent Manner
Colocalizes With Fc-Gamma-Riib Receptor (Fcgr2b) Or Fcgr3/Cd16 At Membrane Ruffles
Tyrosine Phosphorylation May Also Participate In Membrane Localization
Alternative Antibody Names Anti-Phosphatidylinositol 3 -4 -5-Trisphosphate 5-Phosphatase 1 antibody
Anti-Inositol Polyphosphate-5-Phosphatase D antibody
Anti-Inositol Polyphosphate-5-Phosphatase Of 145 Kda antibody
Anti-Sip-145 antibody
Anti-Phosphatidylinositol 4 -5-Bisphosphate 5-Phosphatase antibody
Anti-Sh2 Domain-Containing Inositol 5'-Phosphatase 1 antibody
Anti-Sh2 Domain-Containing Inositol Phosphatase 1 antibody
Anti-Ship-1 antibody
Anti-P150ship antibody
Anti-Hp51cn antibody
Anti-INPP5D antibody
Anti-SHIP antibody
Anti-SHIP1 antibody

Information sourced from Uniprot.org

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