• Western blot analysis of extracts of various cell lines, using ILF2 antibody (STJ11103661) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using ILF2 Rabbit monoclonal antibody (STJ11103661) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human breast cancer using ILF2 rabbit monoclonal antibody (STJ11103661) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using ILF2 antibody (STJ11103661) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ILF2 antibody (200-300) [S1MR] (STJ11103661)

SKU:
STJ11103661

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ILF2 (200-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ILF2
Gene ID: 3608
Uniprot ID: ILF2_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ILF2 (Q12905).
Immunogen Sequence: SALAAIRHARWFEENASQST VKVLIRLLKDLRIRFPGFEP LTPWILDLLGHYAVMNNPTR QPLALNVAYRRCLQILAAGL FLPGSVGITDPCESGNFRVH T
Post Translational Modifications Ubiquitinated at Lys-45 by CRBN with polyubiquitin chains by the CUL4-RING E3 ligase (CRL4-CRBN) and then degraded by the proteasome.
Function Chromatin-interacting protein that forms a stable heterodimer with interleukin enhancer-binding factor 3/ILF3 and plays a role in several biological processes including transcription, innate immunity or cell growth. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus. Together with ILF3, forms an RNA-binding complex that is required for mitotic progression and cytokinesis by regulating the expression of a cluster of mitotic genes. Mechanistically, competes with STAU1/STAU2-mediated mRNA decay. Also plays a role in the inhibition of various viruses including Japanese encephalitis virus or enterovirus 71. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
Protein Name Interleukin Enhancer-Binding Factor 2
Nuclear Factor Of Activated T-Cells 45 Kda
Database Links Reactome: R-HSA-6798695
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Interleukin Enhancer-Binding Factor 2 antibody
Anti-Nuclear Factor Of Activated T-Cells 45 Kda antibody
Anti-ILF2 antibody
Anti-NF45 antibody
Anti-PRO3063 antibody

Information sourced from Uniprot.org

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