• Western blot analysis of various lysates, using IL4 antibody (STJ11104790) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IL4 antibody (21-140) [S4790RM] (STJ11104790)

SKU:
STJ11104790

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IL4 (21-140) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4790RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Il4
Gene ID: 16189
Uniprot ID: IL4_MOUSE
Immunogen Region: 21-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-140 of mouse IL4 (NP_067258.1).
Immunogen Sequence: HIHGCDKNHLREIIGILNEV TGEGTPCTEMDVPNVLTATK NTTESELVCRASKVLRIFYL KHGKTPCLKKNSSVLMELQR LFRAFRCLDSSISCTMNESK STSLKDFLESLKSIMQMDYS
Function Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6.
Protein Name Interleukin-4
Il-4
B-Cell Igg Differentiation Factor
B-Cell Growth Factor 1
B-Cell Stimulatory Factor 1
Bsf-1
Igg1 Induction Factor
Lymphocyte Stimulatory Factor 1
Database Links Reactome: R-MMU-6785807
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-4 antibody
Anti-Il-4 antibody
Anti-B-Cell Igg Differentiation Factor antibody
Anti-B-Cell Growth Factor 1 antibody
Anti-B-Cell Stimulatory Factor 1 antibody
Anti-Bsf-1 antibody
Anti-Igg1 Induction Factor antibody
Anti-Lymphocyte Stimulatory Factor 1 antibody
Anti-Il4 antibody
Anti-Il-4 antibody

Information sourced from Uniprot.org

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