• Western blot analysis of extracts of various cell lines, using IL36G antibody (STJ112204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of human skin cells using IL36G rabbit polyclonal antibody (STJ112204) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL36G antibody (1-193) (STJ112204)

SKU:
STJ112204

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-193
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of mouse IL36G (NP_705731.2).
Immunogen Sequence: MENNEKKNIVYGSDVEMEHE RAGLFVSSAMFSKHPFSTHI SGRETPDFGEVFDLDQQVWI FRNQALVTVPRSHRVTPVSV TILPCKYPESLEQDKGIAIY LGIQNPDKCLFCKEVNGHPT LLLKEEKILDLYHHPEPMKP FLFYHTRTGGTSTFESVAFP GHYIASSKTGNPIFLTSKKG EYYNINFNLDIKS
Background Enables cytokine activity. Acts upstream of or within negative regulation of myoblast differentiation; negative regulation of myoblast fusion; and positive regulation of interleukin-6 production. Located in extracellular space. Orthologous to human IL36G (interleukin 36 gamma).

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance