• Immunofluorescence analysis of HepG2 cells, using IL-10R alpha Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from HepG2 cells, treated with Na2VO3 0.3nM 40', using IL-10R alpha Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using IL-10R Alpha Polyclonal Antibody

Anti-IL10RA antibody (462-511 aa) (STJ93673)

SKU:
STJ93673

Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Interleukin-10 receptor subunit alpha (462-511 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL10RA
Gene ID: 3587
Uniprot ID: I10R1_HUMAN
Immunogen Region: 462-511 aa
Specificity: IL-10R Alpha Polyclonal Antibody detects endogenous levels of IL-10R Alpha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IL-10R alpha at the amino acid range 462-511
Post Translational Modifications Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation. Ubiquitinated by BTRC.ubiquitination leads to endocytosis and subsequent degradation of IL10RA.
Function Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.
Protein Name Interleukin-10 Receptor Subunit Alpha
Il-10 Receptor Subunit Alpha
Il-10r Subunit Alpha
Il-10ra
Cdw210a
Interleukin-10 Receptor Subunit 1
Il-10r Subunit 1
Il-10r1
Cd Antigen Cd210
Database Links Reactome: R-HSA-6783783
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Interleukin-10 Receptor Subunit Alpha antibody
Anti-Il-10 Receptor Subunit Alpha antibody
Anti-Il-10r Subunit Alpha antibody
Anti-Il-10ra antibody
Anti-Cdw210a antibody
Anti-Interleukin-10 Receptor Subunit 1 antibody
Anti-Il-10r Subunit 1 antibody
Anti-Il-10r1 antibody
Anti-Cd Antigen Cd210 antibody
Anti-IL10RA antibody
Anti-IL10R antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance