• STJ70852 (3ug/ml) staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70852 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70852 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ70852 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. This data is from a previous batch, not on sale.

Anti-IFT88/Polaris antibody (C-Term) (STJ70852)

SKU:
STJ70852-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-IFT88/Polaris (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:8000.
WB: Approx. 80kDa band observed in lysates of cell lines THP-1, K562 and KNRK and 85kDa in lysates of cell line U251 (calculated MW of 94.3kDa according to Human NP_783195.2) and 93.1kDa ac
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: IFT88
Gene ID: 8100
Uniprot ID: IFT88_HUMAN
Immunogen Region: C-Term
Accession Number: NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1
Specificity: This antibody is expected to recognise many reported isoforms.
Immunogen Sequence: KKRIDEDDFADEE
Function Positively regulates primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Protein Name Intraflagellar Transport Protein 88 Homolog
Recessive Polycystic Kidney Disease Protein Tg737 Homolog
Tetratricopeptide Repeat Protein 10
Tpr Repeat Protein 10
Database Links Reactome: R-HSA-5610787
Reactome: R-HSA-5620924
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cell Projection
Cilium
Cilium Basal Body
Flagellum
Colocalizes With Entr1 And Gamma-Tubulin At The Basal Body Of Primary Cilia
Colocalizes With Entr1 And Pericentrin At The Centrosome
In Sperm Cells
Localizes To The Manchette
Head-Tail Coupling Apparatus And Flagellum
Alternative Antibody Names Anti-Intraflagellar Transport Protein 88 Homolog antibody
Anti-Recessive Polycystic Kidney Disease Protein Tg737 Homolog antibody
Anti-Tetratricopeptide Repeat Protein 10 antibody
Anti-Tpr Repeat Protein 10 antibody
Anti-IFT88 antibody
Anti-TG737 antibody
Anti-TTC10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance